Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice

Bharath Rathnakar; Vijendra Prabhu; Bola Sadashiva Satish Rao; Subhash Chandra; Sharada Rai; Krishna Kishore Mahato

doi:10.1117/12.2242919

9 December 2016 Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice

Bharath Rathnakar, Vijendra Prabhu, Bola Sadashiva Satish Rao, Subhash Chandra, Sharada Rai, Krishna Kishore Mahato

Proceedings Volume 10013, SPIE BioPhotonics Australasia; 100132Z (2016) https://doi.org/10.1117/12.2242919
Event: SPIE BioPhotonics Australasia, 2016, Adelaide, Australia

Abstract

The present study intends to understand the importance of cellular marker in tissue regeneration regulated upon irradiation of low power laser light in burn inflicted mice. Under anesthetic conditions, the thermal injury was induced on Swiss albino mice of either sex. Following injury, the animals were randomly divided into three groups; i. e., un-illuminated control, the group treated with 5% Povidone iodine (reference standard) and single exposure of 3 J/cm² (830 nm). Burn tissue samples from each group were excised at day 6 post burn injury upon euthanization and used for histological and immunohistochemical analysis. Haematoxylin and Eosin (H and E) staining was performed on the selected sections to asses proliferation and angiogenesis at day 6 post-injury. For immunohistochemical analysis, tissue sections from all the three treatment groups on day 6 were stained using specific antibody against Proliferating cell nuclear antigen (PCNA). The results of the histological and immunohistochemical analysis showed improved tissue restoration in animals treated with optimal laser influence as compared to un-illuminated controls. The findings of present study clearly demonstrated the beneficial effects of 830 nm laser in burn wound healing and its influence in regulating the cellular marker.

Citation Download Citation

Bharath Rathnakar, Vijendra Prabhu, Bola Sadashiva Satish Rao, Subhash Chandra, Sharada Rai, and Krishna Kishore Mahato "Regulation of cellular marker modulated upon irradiation of low power laser light in burn injured mice", Proc. SPIE 10013, SPIE BioPhotonics Australasia, 100132Z (9 December 2016); https://doi.org/10.1117/12.2242919

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available