Rare-earth doped nanocomposites enable multiscale targeted short-wave infrared imaging of metastatic breast cancer

Mark C. Pierce; Laura M. Higgins; Vidya Ganapathy; Harini Kantamneni; Richard E. Riman; Charles M. Roth; Prabhas V. Moghe

doi:10.1117/12.2253511

8 February 2017 Rare-earth doped nanocomposites enable multiscale targeted short-wave infrared imaging of metastatic breast cancer

Mark C. Pierce, Laura M. Higgins, Vidya Ganapathy, Harini Kantamneni, Richard E. Riman, Charles M. Roth, Prabhas V. Moghe

Author Affiliations +

Proceedings Volume 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System; 100430Z (2017) https://doi.org/10.1117/12.2253511
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We are investigating the ability of targeted rare earth (RE) doped nanocomposites to detect and track micrometastatic breast cancer lesions to distant sites in pre-clinical in vivo models. Functionalizing RE nanocomposites with AMD3100 promotes targeting to CXCR4, a recognized marker for highly metastatic disease. Mice were inoculated with SCP-28 (CXCR4 positive) and 4175 (CXCR4 negative) cell lines. Whole animal in vivo SWIR fluorescence imaging was performed after bioluminescence imaging confirmed tumor burden in the lungs. Line-scanning confocal fluorescence microscopy provided high-resolution imaging of RE nanocomposite uptake and native tissue autofluorescence in ex vivo lung specimens. Co-registered optical coherence tomography imaging allowed assessment of tissue microarchitecture. In conclusion, multiscale optical molecular imaging can be performed in pre-clinical models of metastatic breast cancer, using targeted RE-doped nanocomposites.

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Mark C. Pierce, Laura M. Higgins, Vidya Ganapathy, Harini Kantamneni, Richard E. Riman, Charles M. Roth, and Prabhas V. Moghe "Rare-earth doped nanocomposites enable multiscale targeted short-wave infrared imaging of metastatic breast cancer", Proc. SPIE 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System, 100430Z (8 February 2017); https://doi.org/10.1117/12.2253511

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