Characterizing motility dynamics in human RPE cells

Zhuolin Liu; Kazuhiro Kurokawa; Furu Zhang; Donald T. Miller

doi:10.1117/12.2256144

8 February 2017 Characterizing motility dynamics in human RPE cells

Zhuolin Liu, Kazuhiro Kurokawa, Furu Zhang, Donald T. Miller

Proceedings Volume 10045, Ophthalmic Technologies XXVII; 1004515 (2017) https://doi.org/10.1117/12.2256144
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Retinal pigment epithelium (RPE) cells are vital to health of the outer retina, however, are often compromised in ageing and ocular diseases that lead to blindness. Early manifestation of RPE disruption occurs at the cellular level, but while in vivo biomarkers at this scale hold considerable promise, RPE cells have proven extremely challenging to image in the living human eye. Recently we addressed this problem by using organelle motility as a novel contrast agent to enhance the RPE cell in conjunction with 3D resolution of adaptive optics-optical coherence tomography (AO-OCT) to section the RPE layer. In this study, we expand on the central novelty of our method – organelle motility – by characterizing the dynamics of the motility in individual RPE cells, important because of its direct link to RPE physiology. To do this, AO-OCT videos of the same retinal patch were acquired at approximately 1 min intervals or less, time stamped, and registered in 3D with sub-cellular accuracy. Motility was quantified by an exponential decay time constant, the time for motility to decorrelate the speckle field across an RPE cell. In two normal subjects, we found the decay time constant to be just 3 seconds, thus indicating rapid motility in normal RPE cells.

Citation Download Citation

Zhuolin Liu, Kazuhiro Kurokawa, Furu Zhang, and Donald T. Miller "Characterizing motility dynamics in human RPE cells", Proc. SPIE 10045, Ophthalmic Technologies XXVII, 1004515 (8 February 2017); https://doi.org/10.1117/12.2256144

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available