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10 February 2017 Computational method for multi-modal microscopy based on transport of intensity equation
Jiaji Li, Qian Chen, Jiasong Sun, Jialin Zhang, Chao Zuo
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Proceedings Volume 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016); 102501L (2017) https://doi.org/10.1117/12.2266905
Event: Fourth International Conference on Optical and Photonics Engineering, 2016, Chengdu, China
Abstract
In this paper, we develop the requisite theory to describe a hybrid virtual-physical multi-modal imaging system which yields quantitative phase, Zernike phase contrast, differential interference contrast (DIC), and light field moment imaging simultaneously based on transport of intensity equation(TIE). We then give the experimental demonstration of these ideas by time-lapse imaging of live HeLa cell mitosis. Experimental results verify that a tunable lens based TIE system, combined with the appropriate post-processing algorithm, can achieve a variety of promising imaging modalities in parallel with the quantitative phase images for the dynamic study of cellular processes.
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Jiaji Li, Qian Chen, Jiasong Sun, Jialin Zhang, and Chao Zuo "Computational method for multi-modal microscopy based on transport of intensity equation", Proc. SPIE 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016), 102501L (10 February 2017); https://doi.org/10.1117/12.2266905
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KEYWORDS
Microscopy
Computational imaging
Phase retrieval
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