Development and design of up-to-date laser scanning two-photon microscope using in neuroscience

Maxim Doronin; Alexander Popov

doi:10.1117/12.2267160

10 February 2017 Development and design of up-to-date laser scanning two-photon microscope using in neuroscience

Maxim Doronin, Alexander Popov

Proceedings Volume 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016); 102501M (2017) https://doi.org/10.1117/12.2267160
Event: Fourth International Conference on Optical and Photonics Engineering, 2016, Chengdu, China

Abstract

Today one of the main areas of application of two-photon microscopy is biology. This is due to the fact that this technique allows to obtain 3D images of tissues due to laser focus change, that is possible due to substantially greater penetration depth on the main wavelength into biological tissues. Self-developed microscopy system provides possibility to service it and modify the structure of microscope depending on highly specialized experimental design and scientific goals. This article may be regarded as a quick reference to laboratory staff who are wishing to develop their own microscopy system for self-service and modernization of the system and in order to save the lab budget.

Citation Download Citation

Maxim Doronin and Alexander Popov "Development and design of up-to-date laser scanning two-photon microscope using in neuroscience", Proc. SPIE 10250, International Conference on Optical and Photonics Engineering (icOPEN 2016), 102501M (10 February 2017); https://doi.org/10.1117/12.2267160

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