Multimodal fluorescence and photoacoustic microscopy in the frequency domain

Gregor Langer; Bianca Buchegger; Jaroslaw Jacak; Karoline Pfeffer; Thomas A. Klar; Thomas Berer

doi:10.1117/12.2251796

15 February 2017 Multimodal fluorescence and photoacoustic microscopy in the frequency domain

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Karoline Pfeffer, Thomas A. Klar, Thomas Berer

Proceedings Volume 10057, Multimodal Biomedical Imaging XII; 1005707 (2017) https://doi.org/10.1117/12.2251796
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

In this paper a multimodal optical resolution microscope in frequency domain is introduced. Fluorescence and photoacoustic signals are generated simultaneously using an amplitude modulated diode laser. The resulting signals are recorded by a lock-in amplifier. By scanning the sample, two-dimensional plots of the fluorescence and photoacoustic signals can be generated. In this paper, the signal-to-noise ratios of amplitude modulated signals are compared to those expected for pulsed excitation under the assumption that the maximum permissible exposure limits are fulfilled. Furthermore, it is described how to determine the excited state life-times from the measured frequency responses of the fluorescence or photoacoustic signals, respectively.

Citation Download Citation

Gregor Langer, Bianca Buchegger, Jaroslaw Jacak, Karoline Pfeffer, Thomas A. Klar, and Thomas Berer "Multimodal fluorescence and photoacoustic microscopy in the frequency domain", Proc. SPIE 10057, Multimodal Biomedical Imaging XII, 1005707 (15 February 2017); https://doi.org/10.1117/12.2251796

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