UV fluorescence excitation spectroscopy as a non-invasive predictor of epidermal proliferation and clinical performance of cosmetic formulations

Robert Maidhof; Frank Liebel; Cheng Hwang; Eduardo Ruvolo; John Lyga

doi:10.1117/12.2254150

17 February 2017 UV fluorescence excitation spectroscopy as a non-invasive predictor of epidermal proliferation and clinical performance of cosmetic formulations

Robert Maidhof, Frank Liebel, Cheng Hwang, Eduardo Ruvolo, John Lyga

Author Affiliations +

Proceedings Volume 10059, Optical Tomography and Spectroscopy of Tissue XII; 100591V (2017) https://doi.org/10.1117/12.2254150
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The epidermis is the outermost layer of skin and is composed of cells primarily containing keratin. It consists of about ten layers of living cells (keratinocytes) and ten layers of dead cells (corneocytes). These cells are continually shed from the outside and replaced from the inside in a process called desquamation which is controlled by two biological events – proliferation and differentiation.

One method to non-invasively study biological changes in the skin is using fluorescence excitation spectroscopy. Several characteristic excitation-emission peaks occur in skin that have been related to the epidermal and dermal composition. The magnitude of the peak that occurs at 295nm excitation (F295) has been linked to changes in skin proliferation, cell turnover, epidermal thickening, and skin aging. We hypothesize that changes in this fluorescent signal could be used to assess the potential activity of cosmetic anti-aging compounds to deliver a benefit to skin.

Previous work with retinol and glycolic acid, two commonly used actives that effect epidermal proliferation and exfoliation, has demonstrated an increase in F295 (attributed to tryptophan excitation fluorescence). In this study we present the results of a placebo controlled study that aims to correlate changes in F295 with biological performance (epidermal thickening and Ki67 expression).

Citation Download Citation

Robert Maidhof, Frank Liebel, Cheng Hwang, Eduardo Ruvolo, and John Lyga "UV fluorescence excitation spectroscopy as a non-invasive predictor of epidermal proliferation and clinical performance of cosmetic formulations", Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 100591V (17 February 2017); https://doi.org/10.1117/12.2254150

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