Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern

Tongxin Li; Feng Gao; Weiting Chen; Caixia Qi; Panpan Yan; Huijuan Zhao

doi:10.1117/12.2250474

17 February 2017 Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern

Tongxin Li, Feng Gao, Weiting Chen, Caixia Qi, Panpan Yan, Huijuan Zhao

Proceedings Volume 10059, Optical Tomography and Spectroscopy of Tissue XII; 100591Y (2017) https://doi.org/10.1117/12.2250474
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We present a wide-field fluorescence tomography with epi-illumination of sinusoidal pattern. In this scheme, a DMD projector is employed as a spatial light modulator to generate independently wide-field sinusoidal illumination patterns at varying spatial frequencies on a sample, and then the emitted photons at the sample surface were captured with a EM-CCD camera. This method results in a significantly reduced number of the optical field measurements as compared to the point-source-scanning ones and thereby achieves a fast data acquisition that is desired for a dynamic imaging application. Fluorescence yield images are reconstructed using the normalized-Born formulated inversion of the diffusion model. Experimental reconstructions are presented on a phantom embedding the fluorescent targets and compared for a combination of the multiply frequencies. The results validate the ability of the method to determine the target relative depth and quantification with an increasing accuracy.

Citation Download Citation

Tongxin Li, Feng Gao, Weiting Chen, Caixia Qi, Panpan Yan, and Huijuan Zhao "Wide-field fluorescence diffuse optical tomography with epi-illumination of sinusoidal pattern", Proc. SPIE 10059, Optical Tomography and Spectroscopy of Tissue XII, 100591Y (17 February 2017); https://doi.org/10.1117/12.2250474

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