Plasmonic SERS nanochips and nanoprobes for medical diagnostics and bio-energy applications

Hoan T. Ngo; Hsin-Neng Wang; Bridget M. Crawford; Andrew M. Fales; Tuan Vo-Dinh

doi:10.1117/12.2267857

17 February 2017 Plasmonic SERS nanochips and nanoprobes for medical diagnostics and bio-energy applications

Hoan T. Ngo, Hsin-Neng Wang, Bridget M. Crawford, Andrew M. Fales, Tuan Vo-Dinh

Proceedings Volume 10080, Plasmonics in Biology and Medicine XIV; 100800A (2017) https://doi.org/10.1117/12.2267857
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The development of rapid, easy-to-use, cost-effective, high accuracy, and high sensitive DNA detection methods for molecular diagnostics has been receiving increasing interest. Over the last five years, our laboratory has developed several chip-based DNA detection techniques including the molecular sentinel-on-chip (MSC), the multiplex MSC, and the inverse molecular sentinel-on-chip (iMS-on-Chip). In these techniques, plasmonic surface-enhanced Raman scattering (SERS) Nanowave chips were functionalized with DNA probes for single-step DNA detection. Sensing mechanisms were based on hybridization of target sequences and DNA probes, resulting in a distance change between SERS reporters and the Nanowave chip’s gold surface. This distance change resulted in change in SERS intensity, thus indicating the presence and capture of the target sequences. Our techniques were single-step DNA detection techniques. Target sequences were detected by simple delivery of sample solutions onto DNA probe-functionalized Nanowave chips and SERS signals were measured after 1h - 2h incubation. Target sequence labeling or washing to remove unreacted components was not required, making the techniques simple, easy-to-use, and cost effective. The usefulness of the techniques for medical diagnostics was illustrated by the detection of genetic biomarkers for respiratory viral infection and of dengue virus 4 DNA.

Conference Presentation

Citation Download Citation

Hoan T. Ngo, Hsin-Neng Wang, Bridget M. Crawford, Andrew M. Fales, and Tuan Vo-Dinh "Plasmonic SERS nanochips and nanoprobes for medical diagnostics and bio-energy applications", Proc. SPIE 10080, Plasmonics in Biology and Medicine XIV, 100800A (17 February 2017); https://doi.org/10.1117/12.2267857

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