Monitoring of interstitial buffer systems using micro-dialysis and infrared spectrometry

H. M. Heise; L. Cocchieri; T. Vahlsing; D. Ihrig; J. Elm

doi:10.1117/12.2253139

17 February 2017 Monitoring of interstitial buffer systems using micro-dialysis and infrared spectrometry

H. M. Heise, L. Cocchieri, T. Vahlsing, D. Ihrig, J. Elm

Proceedings Volume 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics; 100720E (2017) https://doi.org/10.1117/12.2253139
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Nowadays, continuous sensing systems are important point-of-care devices for the hospital and personalized patient technology. FTIR-spectrometers have been successfully employed for the development of bed-side systems. In-vivo applications for critically ill patients can be envisaged for analytes and parameters, which are of interest for intensive care such as lactate, urea, pCO₂ and pH. The human body maintains the blood pH around 7.4, but for severe pH level changes acidosis or alkalosis can lead to serious health problems. Three different buffer systems exist based on bicarbonate, phosphate and proteins; for the most important bicarbonate and phosphate systems infrared transmission spectra were recorded. By using the CO₂ and HCO₃ - bands of the bicarbonate spectra, the pH of the harvested biofluid can be predicted using the Henderson-Hasselbalch equation. Furthermore, we studied the solubility of CO₂ in aqueous solutions using gas mixtures of N₂ and CO₂ with known composition within partial pressures of CO₂ as relevant for invivo conditions. Thus, values of pCO₂ up to 150 mm Hg (200 hPa) with distilled water and a Ringer solution, which is an isotonic electrolyte solution used for medical infusion, were measured at 25 °C and 37 °C (normal body temperature).

Conference Presentation

Citation Download Citation

H. M. Heise, L. Cocchieri, T. Vahlsing, D. Ihrig, and J. Elm "Monitoring of interstitial buffer systems using micro-dialysis and infrared spectrometry", Proc. SPIE 10072, Optical Diagnostics and Sensing XVII: Toward Point-of-Care Diagnostics, 100720E (17 February 2017); https://doi.org/10.1117/12.2253139

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