Molecular counting of membrane receptor subunits with single-molecule localization microscopy

Carmen Krüger; Franziska Fricke; Christos Karathanasis; Marina S. Dietz; Sebastian Malkusch; Gerhard Hummer; Mike Heilemann

doi:10.1117/12.2256638

21 February 2017 Molecular counting of membrane receptor subunits with single-molecule localization microscopy

Carmen Krüger, Franziska Fricke, Christos Karathanasis, Marina S. Dietz, Sebastian Malkusch, Gerhard Hummer, Mike Heilemann

Author Affiliations +

Proceedings Volume 10071, Single Molecule Spectroscopy and Superresolution Imaging X; 100710K (2017) https://doi.org/10.1117/12.2256638
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

We report on quantitative single-molecule localization microscopy, a method that next to super-resolved images of cellular structures provides information on protein copy numbers in protein clusters. This approach is based on the analysis of blinking cycles of single fluorophores, and on a model-free description of the distribution of the number of blinking events. We describe the experimental and analytical procedures, present cellular data of plasma membrane proteins and discuss the applicability of this method.

Citation Download Citation

Carmen Krüger, Franziska Fricke, Christos Karathanasis, Marina S. Dietz, Sebastian Malkusch, Gerhard Hummer, and Mike Heilemann "Molecular counting of membrane receptor subunits with single-molecule localization microscopy", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710K (21 February 2017); https://doi.org/10.1117/12.2256638

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