Imaging cytochrome C oxidase and FoF1-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy

Franziska Foertsch; Mykhailo Ilchenko; Thomas Heitkamp; Silke Noßmann; Birgit Hoffmann; Ilka Starke; Ralf Mrowka; Christoph Biskup; Michael Börsch

doi:10.1117/12.2251210

21 February 2017 Imaging cytochrome C oxidase and F_oF₁-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy

Franziska Foertsch, Mykhailo Ilchenko, Thomas Heitkamp, Silke Noßmann, Birgit Hoffmann, Ilka Starke, Ralf Mrowka, Christoph Biskup, Michael Börsch

Author Affiliations +

Proceedings Volume 10071, Single Molecule Spectroscopy and Superresolution Imaging X; 100710P (2017) https://doi.org/10.1117/12.2251210
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Cytochrome C oxidase and F_oF₁-ATP synthase constitute complex IV and V, respectively, of the five membrane-bound enzymes in mitochondria comprising the respiratory chain. These enzymes are located in the inner mitochondrial membrane (IMM), which exhibits large invaginations called cristae. According to recent electron cryotomography, FoF1-ATP synthases are located predominantly at the rim of the cristae, while cytochrome C oxidases are likely distributed in planar membrane areas of the cristae. Previous FLIM measurements (K. Busch and coworkers) of complex II and III unravelled differences in the local environment of the membrane enzymes in the cristae. Here, we tagged complex IV and V with mNeonGreen and investigated their mitochondrial nano-environment by FLIM and superresolution microscopy in living human cells. Different lifetimes and anisotropy values were found and will be discussed.

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Franziska Foertsch, Mykhailo Ilchenko, Thomas Heitkamp, Silke Noßmann, Birgit Hoffmann, Ilka Starke, Ralf Mrowka, Christoph Biskup, and Michael Börsch "Imaging cytochrome C oxidase and F_oF₁-ATP synthase in mitochondrial cristae of living human cells by FLIM and superresolution microscopy", Proc. SPIE 10071, Single Molecule Spectroscopy and Superresolution Imaging X, 100710P (21 February 2017); https://doi.org/10.1117/12.2251210

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