Quantitative assessment of cancer cell morphology and movement using telecentric digital holographic microscopy

Thanh C. Nguyen; George Nehmetallah; Van Lam; Byung Min Chung; Christopher Raub

doi:10.1117/12.2256128

21 February 2017 Quantitative assessment of cancer cell morphology and movement using telecentric digital holographic microscopy

Thanh C. Nguyen, George Nehmetallah, Van Lam, Byung Min Chung, Christopher Raub

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 100740U (2017) https://doi.org/10.1117/12.2256128
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Digital holographic microscopy (DHM) provides label-free and real-time quantitative phase information relevant to the analysis of dynamic biological systems. A DHM based on telecentric configuration optically mitigates phase aberrations due to the microscope objective and linear high frequency fringes due to the reference beam thus minimizing digital aberration correction needed for distortion free 3D reconstruction. The purpose of this work is to quantitatively assess growth and migratory behavior of invasive cancer cells using a telecentric DHM system. Together, the height and lateral shape features of individual cells, determined from time-lapse series of phase reconstructions, should reveal aspects of cell migration, cell-matrix adhesion, and cell cycle phase transitions. To test this, MDA-MB-231 breast cancer cells were cultured on collagen-coated or un-coated glass, and 3D holograms were reconstructed over 2 hours. Cells on collagencoated glass had an average 14% larger spread area than cells on uncoated glass (n=18-22 cells/group). The spread area of cells on uncoated glass were 15-21% larger than cells seeded on collagen hydrogels (n=18-22 cells/group). Premitotic cell rounding was observed with average phase height increasing 57% over 10 minutes. Following cell division phase height decreased linearly (R²=0.94) to 58% of the original height pre-division. Phase objects consistent with lamellipodia were apparent from the reconstructions at the leading edge of migrating cells. These data demonstrate the ability to track quantitative phase parameters and relate them to cell morphology during cell migration and division on adherent substrates, using telecentric DHM. The technique enables future studies of cell-matrix interactions relevant to cancer.

Conference Presentation

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Thanh C. Nguyen, George Nehmetallah, Van Lam, Byung Min Chung, and Christopher Raub "Quantitative assessment of cancer cell morphology and movement using telecentric digital holographic microscopy", Proc. SPIE 10074, Quantitative Phase Imaging III, 100740U (21 February 2017); https://doi.org/10.1117/12.2256128

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