Monitoring of live cell cultures during apoptosis by phase imaging and Raman spectroscopy

Anna Sharikova; George Saide; Lauren Sfakis; Jun Yong Park; Habben Desta; Maxwell C. Maloney; James Castracane; Supriya D. Mahajan; Alexander Khmaladze

doi:10.1117/12.2256134

21 February 2017 Monitoring of live cell cultures during apoptosis by phase imaging and Raman spectroscopy

Anna Sharikova, George Saide, Lauren Sfakis, Jun Yong Park, Habben Desta, Maxwell C. Maloney, James Castracane, Supriya D. Mahajan, Alexander Khmaladze

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 100740V (2017) https://doi.org/10.1117/12.2256134
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Non-invasive live cell measurements are an important tool in biomedical research. We present a combined digital holography/Raman spectroscopy technique to study live cell cultures during apoptosis. Digital holographic microscopy records an interference pattern between object and reference waves, so that the computationally reconstructed holographic image contains both amplitude and phase information about the sample. When the phase is mapped across the sample and converted into height information for each pixel, a three dimensional image is obtained. The measurement of live cell cultures by digital holographic microscopy yields information about cell shape and volume, changes to which are reflective of alterations in cell cycle and initiation of cell death mechanisms. Raman spectroscopy, on the other hand, is sensitive to rotational and vibrational molecular transitions, as well as intermolecular vibrations. Therefore, Raman spectroscopy provides complementary information about cells, such as protein, lipid and nucleic acid content, and, particularly, the spectral signatures associated with structural changes in molecules. The cell cultures are kept in the temperature-controlled environmental chamber during the experiment, which allows monitoring over multiple cell cycles. The DHM system combines a visible (red) laser source with conventional microscope base, and LabVIEW-run data processing. We analyzed and compared cell culture information obtained by these two methods.

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Anna Sharikova, George Saide, Lauren Sfakis, Jun Yong Park, Habben Desta, Maxwell C. Maloney, James Castracane, Supriya D. Mahajan, and Alexander Khmaladze "Monitoring of live cell cultures during apoptosis by phase imaging and Raman spectroscopy", Proc. SPIE 10074, Quantitative Phase Imaging III, 100740V (21 February 2017); https://doi.org/10.1117/12.2256134

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