Micro patterned surfaces: an effective tool for long term digital holographic microscopy cell imaging

Sarah Mues; Inga Lilge; Holger Schönherr; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2253280

21 February 2017 Micro patterned surfaces: an effective tool for long term digital holographic microscopy cell imaging

Sarah Mues, Inga Lilge, Holger Schönherr, Björn Kemper, Jürgen Schnekenburger

Proceedings Volume 10074, Quantitative Phase Imaging III; 1007410 (2017) https://doi.org/10.1117/12.2253280
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The major problem of Digital Holographic Microscopy (DHM) long term live cell imaging is that over time most of the tracked cells move out of the image area and other ones move in. Therefore, most of the cells are lost for the evaluation of individual cellular processes. Here, we present an effective solution for this crucial problem of long-term microscopic live cell analysis. We have generated functionalized slides containing areas of 250 μm per 200 μm. These micropatterned biointerfaces consist of passivating polyaclrylamide brushes (PAAm). Inner areas are backfilled with octadecanthiol (ODT), which allows cell attachment. The fouling properties of these surfaces are highly controllable and therefore the defined areas designed for the size our microscopic image areas were effective in keeping all cells inside the rectangles over the selected imaging period.

Conference Presentation

Citation Download Citation

Sarah Mues, Inga Lilge, Holger Schönherr, Björn Kemper, and Jürgen Schnekenburger "Micro patterned surfaces: an effective tool for long term digital holographic microscopy cell imaging", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007410 (21 February 2017); https://doi.org/10.1117/12.2253280

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