In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system

Chih-Ju Lin; Ning Kang; Jian-Ye Lee; Hsuan-Shu Lee; Chen-Yuan Dong

doi:10.1117/12.2254944

21 February 2017 In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system

Chih-Ju Lin, Ning Kang, Jian-Ye Lee, Hsuan-Shu Lee, Chen-Yuan Dong

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100691J (2017) https://doi.org/10.1117/12.2254944
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Liver performs xenobiotic excretion out of hepatocytes with metabolic function. However, hepatocellular metabolism was non-uniform in hepatocyte. Hepatocellular metabolism could be different in nucleus and cytoplasm. In this study, we use the molecular probe 6-carboxyfluorescein diacetate (6-CFDA) to simulate xenobiotic metabolism in hepatocytes with multi-photon fluorescence microscopy in vivo. 6-CFDA was processed by intracellular esterase to 6- carboxyfluorescein (6-CF) with green fluorescence. And this probe was used to study differences in cytoplasmic and nuclear metabolism of hepatocytes.

Citation Download Citation

Chih-Ju Lin, Ning Kang, Jian-Ye Lee, Hsuan-Shu Lee, and Chen-Yuan Dong "In vivo multiphoton imaging and quantification of cytoplasmic and nuclear metabolism in the hepatobiliary system", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691J (21 February 2017); https://doi.org/10.1117/12.2254944

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