Genetically encoded sensors and fluorescence microscopy for anticancer research

Elena V. Zagaynova M.D.; Marina V. Shirmanova; Tatiana F. Sergeeva; Natalia V. Klementieva; Alexander S. Mishin; Alena I. Gavrina; Olga A. Zlobovskay; Olga E. Furman; Varvara V. Dudenkova; Gregory S. Perelman; Maria M. Lukina; Konstantin A. Lukyanov

doi:10.1117/12.2252151

21 February 2017 Genetically encoded sensors and fluorescence microscopy for anticancer research

Elena V. Zagaynova M.D., Marina V. Shirmanova, Tatiana F. Sergeeva, Natalia V. Klementieva, Alexander S. Mishin, Alena I. Gavrina, Olga A. Zlobovskay, Olga E. Furman, Varvara V. Dudenkova, Gregory S. Perelman, Maria M. Lukina, Konstantin A. Lukyanov

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Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100691K (2017) https://doi.org/10.1117/12.2252151
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Early response of cancer cells to chemical compounds and chemotherapeutic drugs were studied using novel fluorescence tools and microscopy techniques. We applied confocal microscopy, two-photon fluorescence lifetime imaging microscopy and super-resolution localization-based microscopy to assess structural and functional changes in cancer cells in vitro. The dynamics of energy metabolism, intracellular pH, caspase-3 activation during staurosporine-induced apoptosis as well as actin cytoskeleton rearrangements under chemotherapy were evaluated. We have showed that new genetically encoded sensors and advanced fluorescence microscopy methods provide an efficient way for multiparameter analysis of cell activities

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Elena V. Zagaynova M.D., Marina V. Shirmanova, Tatiana F. Sergeeva, Natalia V. Klementieva, Alexander S. Mishin, Alena I. Gavrina, Olga A. Zlobovskay, Olga E. Furman, Varvara V. Dudenkova, Gregory S. Perelman, Maria M. Lukina, and Konstantin A. Lukyanov "Genetically encoded sensors and fluorescence microscopy for anticancer research", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691K (21 February 2017); https://doi.org/10.1117/12.2252151

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