980 nm and 808 nm excitable upconversion nanoparticles for the detection of enzyme related reactions

Sandy F Himmelstoß; Lisa M. Wiesholler; Markus Buchner; Verena Muhr; Susanne Märkl; Antje J. Baeumner; Thomas Hirsch

doi:10.1117/12.2252381

22 February 2017 980 nm and 808 nm excitable upconversion nanoparticles for the detection of enzyme related reactions

Sandy F Himmelstoß, Lisa M. Wiesholler, Markus Buchner, Verena Muhr, Susanne Märkl, Antje J. Baeumner, Thomas Hirsch

Proceedings Volume 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV; 100770L (2017) https://doi.org/10.1117/12.2252381
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Upconverting luminescent nanoparticles (UCNPs) represent an interesting class of nanomaterials for bioanalytical applications. Due to their excitation in the near infrared region of the spectra, no fluorescence of biological compounds is trigged. Compared to other nanomaterials like quantum dots they exhibit low cytotoxicity, high photostability, no blinking and chemical inertness. Nevertheless, UCNPs suffer from low quantum efficiency. Here we report on two different core-shell particle systems which have a core consisting of NaYF₄ doped with Yb³⁺/ Tm³⁺ and an additional inert shell (NaYF₄) or an active shell (NaYF₄ doped with Yb³⁺/Nd³⁺). Nanoparticles without Yb³⁺ as sensitizer can be excited at 980 nm. However, water has an absorption band in this region. This results in a reduction of the upconversion efficiency in aqueous systems and a heating of the solution. For bioanalytical application, more beneficial is the shifting of the wavelength to 808 nm by additional doping of the shell with Nd³⁺. Both core-shell systems were investigated in respect to the monitor enzymatic reactions of dehydrogenases and oxidases involving the generation of either NADH or FADH₂.

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Sandy F Himmelstoß, Lisa M. Wiesholler, Markus Buchner, Verena Muhr, Susanne Märkl, Antje J. Baeumner, and Thomas Hirsch "980 nm and 808 nm excitable upconversion nanoparticles for the detection of enzyme related reactions", Proc. SPIE 10077, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XIV, 100770L (22 February 2017); https://doi.org/10.1117/12.2252381

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