‘Giant’ nanocrystal quantum dots (gNQDs) as FRET donors

Margaret Chern; Thuy Nguyen; Allison Dennis

doi:10.1117/12.2261716

22 February 2017 ‘Giant’ nanocrystal quantum dots (gNQDs) as FRET donors

Margaret Chern, Thuy Nguyen, Allison Dennis

Proceedings Volume 10078, Colloidal Nanoparticles for Biomedical Applications XII; 100780L (2017) https://doi.org/10.1117/12.2261716
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

High-quality core/shell CdSe/xCdS quantum dots (QDs) ranging from 3 to 20 nm in diameter were synthesized for use as Förster Resonance Energy Transfer (FRET) donors. gNQDs are carefully characterized for size, emission, absorption, QY, and brightness in both organic and aqueous solution. FRET has been verified in optimally designed systems that use short capping ligands and donor-acceptor pairs that have well-matched emission and absorption spectra. The interplay between shell thickness, donor-acceptor distance, and particle brightness is systematically analyzed to optimize our biosensor design.

Conference Presentation

Citation Download Citation

Margaret Chern, Thuy Nguyen, and Allison Dennis "‘Giant’ nanocrystal quantum dots (gNQDs) as FRET donors", Proc. SPIE 10078, Colloidal Nanoparticles for Biomedical Applications XII, 100780L (22 February 2017); https://doi.org/10.1117/12.2261716

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