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Proceedings Article

Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields

[+] Author Affiliations
Zachary Coker, Maria Troyanova-Wood, Andrew Traverso, Zhaokai Meng, Charles Ballmann, Georgi Petrov, Vladislav Yakovlev

Texas A&M Univ. (United States)

Bennett L. Ibey

Air Force Research Lab. (United States)

Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 1006604 (February 22, 2017); doi:10.1117/12.2253451
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From Conference Volume 10066

  • Energy-based Treatment of Tissue and Assessment IX
  • Thomas P. Ryan
  • San Francisco, California, United States | January 28, 2017

abstract

Nanosecond electric pulses (nsEPs) are known to cause a variety of effects on mammalian cells, ranging from destabilization of cell membranes to changes in cytoskeleton and elastic moduli. Measurement of a cells mechanoelastic properties have previously been limited to only invasive and destructive techniques such as atomic force microscopy or application of optical tweezers. However, due to recent advances, Brillouin spectroscopy has now become viable as a non-contact, non-invasive method for measuring these properties in cells and other materials. Here, we present progress toward applying Brillouin spectroscopy using a unique microscopy system for measuring changes in CHO-K1 cells when exposed to nsEPs of 600ns pulse duration with intensity of 50kV/cm. Successful measurement of mechanoelastic changes in these cells will demonstrate Brillouin spectroscopy as a viable method for measuring changes in elastic properties of other cells and living organisms. © (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Zachary Coker ; Maria Troyanova-Wood ; Andrew Traverso ; Zhaokai Meng ; Charles Ballmann, et al.
" Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields ", Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 1006604 (February 22, 2017); doi:10.1117/12.2253451; http://dx.doi.org/10.1117/12.2253451


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