Nanosecond electric pulses (nsEPs) are known to cause a variety of effects on mammalian cells, ranging from destabilization of cell membranes to changes in cytoskeleton and elastic moduli. Measurement of a cells mechanoelastic properties have previously been limited to only invasive and destructive techniques such as atomic force microscopy or application of optical tweezers. However, due to recent advances, Brillouin spectroscopy has now become viable as a non-contact, non-invasive method for measuring these properties in cells and other materials. Here, we present progress toward applying Brillouin spectroscopy using a unique microscopy system for measuring changes in CHO-K1 cells when exposed to nsEPs of 600ns pulse duration with intensity of 50kV/cm. Successful measurement of mechanoelastic changes in these cells will demonstrate Brillouin spectroscopy as a viable method for measuring changes in elastic properties of other cells and living organisms.
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Zachary Coker ; Maria Troyanova-Wood ; Andrew Traverso ; Zhaokai Meng ; Charles Ballmann, et al.
Toward investigating changes in cell mechanoelastic properties in response to nanosecond pulsed electric fields
", Proc. SPIE 10066, Energy-based Treatment of Tissue and Assessment IX, 1006604 (February 22, 2017); doi:10.1117/12.2253451; http://dx.doi.org/10.1117/12.2253451