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Proceedings Article

Multimodal imaging of vocal fold scarring in a rabbit model by multiphoton microscopy

[+] Author Affiliations
Alexei Kazarine, Sarah Bouhabel, Annie H. Douillette, Karen Kost, Nicole Y. K. Li-Jessen, Luc Mongeau, Paul W. Wiseman

McGill Univ. (Canada)

Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692E (March 2, 2017); doi:10.1117/12.2250412
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From Conference Volume 10069

  • Multiphoton Microscopy in the Biomedical Sciences XVII
  • Ammasi Periasamy; Peter T. C. So; Karsten König; Xiaoliang S. Xie
  • San Francisco, California, United States | January 28, 2017

abstract

Vocal fold scarring as a result of injury or disease can lead to voice disorders which can significantly affect the quality of life. During the scarring process, the normally elastic tissue of the vocal fold lamina propria is replaced by a much stiffer collagen-based fibrotic tissue, which impacts the fold’s ability to vibrate. Surgical removal of this tissue is often ineffective and can result in further scarring. Injectable biomaterials, a form of tissue engineering, have been proposed as a potential solution to reduce existing scars or prevent scarring altogether. In order to properly evaluate the effectiveness of these new materials, multiphoton microscopy emerges as an effective tool due to its intrinsic multiple label free contrast mechanisms that highlight extracellular matrix elements. In this study, we evaluate the spatial distribution of collagen and elastin fibers in a rabbit model using second harmonic generation (SHG), third harmonic generation (THG) and two photon autofluorescence (TPAF) applied to unlabeled tissue sections. In comparison to traditional methods that rely on histological staining or immunohistochemistry, SHG, THG and TPAF provide a more reliable detection of these native proteins. The evaluation of collagen levels allows us to follow the extent of scarring, while the presence of elastin fibers is thought to be indicative of the level of healing of the injured fold. Using these imaging modalities, we characterize the outcome of injectable biomaterial treatments in order to direct future treatments for tissue engineering. © (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Alexei Kazarine ; Sarah Bouhabel ; Annie H. Douillette ; Karen Kost ; Nicole Y. K. Li-Jessen, et al.
" Multimodal imaging of vocal fold scarring in a rabbit model by multiphoton microscopy ", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692E (March 2, 2017); doi:10.1117/12.2250412; http://dx.doi.org/10.1117/12.2250412


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