Spatial Frequency Modulated Imaging (SPIFI) with amplitude or phase grating from a spatial light modulator

Michael D. Young; Emerson C. Barbano; Nathan Worts; Jeffrey J. Field; Christopher Hoy; Keith A. Wernsing; Randy A. Bartels; Jeff Squier

doi:10.1117/12.2251308

2 March 2017 Spatial Frequency Modulated Imaging (SPIFI) with amplitude or phase grating from a spatial light modulator

Michael D. Young, Emerson C. Barbano, Nathan Worts, Jeffrey J. Field, Christopher Hoy, Keith A. Wernsing, Randy A. Bartels, Jeff Squier

Author Affiliations +

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100692P (2017) https://doi.org/10.1117/12.2251308
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Spatial Frequency Modulated Imaging (SPIFI) with single element detection has previously been demonstrated with a time varying amplitude spatial frequency. This has been shown in a variety of modalities (linear, TPEF, SHG) and also with variations on the base design to provide additional dimensions of information. SPIFI is also capable of providing enhanced resolution images. However, the signal-to-noise is a limiting factor in the quality of the resolution enhancement. We present a microscope design which uses a nematic spatial light modulator to provide a time varying amplitude from an amplitude or phase grating. Twophoton excitation fluorescence images of 10-µm fluorescent polystyrene beads are presented using a phase grating. Additionally, the microscope can provide spatial gratings in polarization which provide an alternative means of imaging in third harmonic generation (THG). THG images are provided using an amplitude and polarization-grating modulation pattern.

Citation Download Citation

Michael D. Young, Emerson C. Barbano, Nathan Worts, Jeffrey J. Field, Christopher Hoy, Keith A. Wernsing, Randy A. Bartels, and Jeff Squier "Spatial Frequency Modulated Imaging (SPIFI) with amplitude or phase grating from a spatial light modulator", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100692P (2 March 2017); https://doi.org/10.1117/12.2251308

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