Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy

Markus Seeger; Angelos Karlas; Dominik Soliman; Jaroslav Pelisek; Vasilis Ntziachristos

doi:10.1117/12.2254891

3 March 2017 Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy

Markus Seeger, Angelos Karlas, Dominik Soliman, Jaroslav Pelisek, Vasilis Ntziachristos

Author Affiliations +

Proceedings Volume 10064, Photons Plus Ultrasound: Imaging and Sensing 2017; 1006455 (2017) https://doi.org/10.1117/12.2254891
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Carotid atheromatosis is causally related to stroke, a leading cause of disability and death. We present the analysis of a human carotid atheroma using a novel hybrid microscopy system that combines optical-resolution optoacoustic (photoacoustic) microscopy and several non-linear optical microscopy modalities (second and third harmonic generation, as well as, two-photon excitation fluorescence) to achieve a multimodal examination of the extracted tissue within the same imaging framework. Our system enables the label-free investigation of atheromatous human carotid tissue with a resolution of about 1 μm and allows for the congruent interrogation of plaque morphology and clinically relevant constituents such as red blood cells, collagen, and elastin. Our data reveal mutual interactions between blood embeddings and connective tissue within the atheroma, offering comprehensive insights into its stage of evolution and severity, and potentially facilitating the further development of diagnostic tools, as well as treatment strategies.

Citation Download Citation

Markus Seeger, Angelos Karlas, Dominik Soliman, Jaroslav Pelisek, and Vasilis Ntziachristos "Hybrid microscopy of human carotid atheroma by means of optical-resolution optoacoustic and non-linear optical microscopy", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 1006455 (3 March 2017); https://doi.org/10.1117/12.2254891

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