Label-free hyperspectral dark-field microscopy for quantitative scatter imaging

Philip Cheney; David McClatchy III; Stephen Kanick; Paul Lemaillet; David Allen; Daniel Samarov; Brian Pogue; Jeeseong Hwang

doi:10.1117/12.2263336

15 March 2017 Label-free hyperspectral dark-field microscopy for quantitative scatter imaging

Philip Cheney, David McClatchy III, Stephen Kanick, Paul Lemaillet, David Allen, Daniel Samarov, Brian Pogue, Jeeseong Hwang

Author Affiliations +

Proceedings Volume 10056, Design and Quality for Biomedical Technologies X; 1005602 (2017) https://doi.org/10.1117/12.2263336
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

A hyperspectral dark-field microscope has been developed for imaging spatially distributed diffuse reflectance spectra from light-scattering samples. In this report, quantitative scatter spectroscopy is demonstrated with a uniform scattering phantom, namely a solution of polystyrene microspheres. A Monte Carlo-based inverse model was used to calculate the reduced scattering coefficients of samples of different microsphere concentrations from wavelength-dependent backscattered signal measured by the dark-field microscope. The results are compared to the measurement results from a NIST double-integrating sphere system for validation. Ongoing efforts involve quantitative mapping of scattering and absorption coefficients in samples with spatially heterogeneous optical properties.

Citation Download Citation

Philip Cheney, David McClatchy III, Stephen Kanick, Paul Lemaillet, David Allen, Daniel Samarov, Brian Pogue, and Jeeseong Hwang "Label-free hyperspectral dark-field microscopy for quantitative scatter imaging", Proc. SPIE 10056, Design and Quality for Biomedical Technologies X, 1005602 (15 March 2017); https://doi.org/10.1117/12.2263336

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