Synchronous fluorescence spectroscopy of colon neoplasia

Ekaterina Borisova; Oxana Semyachkina-Glushkovskaya; Tsanislava Genova; Nikolay Penkov; Ivan Terziev; Borislav Vladimirov; Latchezar Avramov

doi:10.1117/12.2269382

24 March 2017 Synchronous fluorescence spectroscopy of colon neoplasia

Ekaterina Borisova, Oxana Semyachkina-Glushkovskaya, Tsanislava Genova, Nikolay Penkov, Ivan Terziev, Borislav Vladimirov, Latchezar Avramov

Author Affiliations +

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 1033602 (2017) https://doi.org/10.1117/12.2269382
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

Synchronous fluorescence spectroscopy (SFS) is a steady-state approach that we used for evaluation of specific fluorescence characteristics of cancerous colorectal tissues. SFS allow narrowing of the fluorescence spectra received, which increase the spectral resolution and improve the analysis of the fluorescence origin in such complex objects, such as biological tissues. In our study we investigate the characteristic differences, with diagnostic meaning, in the synchronous fluorescence spectra (SFS) of cancerous and healthy colorectal tissues ex vivo using a spectrofluorimeter FluoroLog3 (HORIBA, JobinYvon, France) for obtaining of the SFS data in a broad spectral range (300-800 nm) using excitation in the range of 280-440 nm with a delta lambda between 0 and 200 nm with a 10 nm step between scanning excitation and emission data. The procedure of obtaining the investigated samples ex vivo includes their excision during surgery for removal of neoplasia lesions. After the surgical removal biological samples are transported in isothermal conditions and safekeeping solution from the hospital to the spectral laboratory, where their spectral properties were investigated. All patients received and signed written informed consent and this research is approved by Ethics committee of University Hospital “Tsaritsa Yoanna”, Sofia. Histological analysis was used as “gold standard” for evaluation of tissue samples and comparison of the spectral data received.

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Ekaterina Borisova, Oxana Semyachkina-Glushkovskaya, Tsanislava Genova, Nikolay Penkov, Ivan Terziev, Borislav Vladimirov, and Latchezar Avramov "Synchronous fluorescence spectroscopy of colon neoplasia", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 1033602 (24 March 2017); https://doi.org/10.1117/12.2269382

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