Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezers

Kisung Lee; Alexei Muravyov; Alexei Semenov; Christian Wagner; Alexander Priezzhev; Eugeny Lyubin; Andrey Fedyanin

doi:10.1117/12.2271646

24 March 2017 Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezers

Kisung Lee, Alexei Muravyov, Alexei Semenov, Christian Wagner, Alexander Priezzhev, Eugeny Lyubin, Andrey Fedyanin

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 1033606 (2017) https://doi.org/10.1117/12.2271646
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

The protein contribution to the red blood cell (RBC) aggregation is studied using the in-house made two-channeled optical tweezers. The cells interaction was characterized using two forces: the force required for separating two cells (F_D – disaggregating force) and the force required for holding them from their spontaneous aggregation (F_A – aggregating force). The gamma globulin solutions with/without albumin were used to induce the RBC aggregation. The strong interaction (3-10 pN) between the cells was measured within the contact formed using optical tweezers. We found that F_D becomes stronger as the gamma globulin concentration increases, while the addition of albumin to the solution led to the significant (few fold) enhancement of the cells interaction forces. However, despite of the strong interaction between the cells their spontaneous overlapping was not observed, unlike the case in plasma, where the cells did increase their overlapping surface, when attached with small interacting surface and released from optical traps. This work in addition to our previous work with model solutions of fibrinogen allows us to conclude that the synergy of blood components is one of the most important features that contribute to the reversible RBC aggregation.

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Kisung Lee, Alexei Muravyov, Alexei Semenov, Christian Wagner, Alexander Priezzhev, Eugeny Lyubin, and Andrey Fedyanin "Gamma globulins-induced interaction between two red blood cells: forces measurement with optical tweezers", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 1033606 (24 March 2017); https://doi.org/10.1117/12.2271646

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KEYWORDS

Blood

Optical tweezers

Proteins

Plasma

Glasses

Light emitting diodes

Calibration

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