Different reaction of the core histones H2A and H2B to red laser irradiation

G. E. Brill; A. V. Egorova; I. O. Bugaeva; D. E. Postnov; O. V. Ushakova

doi:10.1117/12.2267949

24 March 2017 Different reaction of the core histones H2A and H2B to red laser irradiation

G. E. Brill, A. V. Egorova, I. O. Bugaeva, D. E. Postnov, O. V. Ushakova

Proceedings Volume 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII; 103360G (2017) https://doi.org/10.1117/12.2267949
Event: Saratov Fall Meeting 2016: Fourth International Symposium on Optics and Biophotonics, 2016, Saratov, Russian Federation

Abstract

Analysis of the influence of red laser irradiation on the processes of self-assembly of the core histones H2A and H2B was performed using a wedge dehydration method. Image-analysis of facies included their qualitative characteristics and calculation of quantitative parameters with subsequent statistical processing. It was established that linearly polarized red laser light (λ - 660 nm, 1 J/cm²) significantly modified the process of self-assembly of core histone H2B, whereas the structure of the facies of H2A histone changed to a lesser extent. Histones were used in the form of aqueous salt solutions. The effect of red light seems to result from the formation of singlet oxygen by direct laser excitation of molecular oxygen.

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G. E. Brill, A. V. Egorova, I. O. Bugaeva, D. E. Postnov, and O. V. Ushakova "Different reaction of the core histones H2A and H2B to red laser irradiation ", Proc. SPIE 10336, Saratov Fall Meeting 2016: Optical Technologies in Biophysics and Medicine XVIII, 103360G (24 March 2017); https://doi.org/10.1117/12.2267949

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