Functional optical imaging of tracheal health (Conference Presentation)

Daniel A. Gil; Joe T. Sharick; Ute A. Gamm; Michael A. Choma; Melissa C. Skala

doi:10.1117/12.2252226

19 April 2017 Functional optical imaging of tracheal health (Conference Presentation)

Daniel A. Gil, Joe T. Sharick, Ute A. Gamm, Michael A. Choma, Melissa C. Skala

Proceedings Volume 10041, Optical Techniques in Pulmonary Medicine II; 100410O (2017) https://doi.org/10.1117/12.2252226
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The health of the tracheal mucosa is an important, but poorly understood, aspect of critical care medicine. Many critical care patients are mechanically ventilated through an endotracheal tube that can cause local inflammation and blunt damage to the ciliated epithelial cells lining the trachea. These cilia clear mucus and infectious agents from the respiratory tract, so impaired ciliary function may lead to increased susceptibility to respiratory infection. Therefore, a minimally-invasive method to monitor mucosal health and ciliary function in intubated patients would be valuable to critical care medicine. Optical metabolic imaging (OMI) can quantitatively assess the metabolic state of cells by measuring the fluorescence intensities of endogenous metabolic co-enzymes NAD(P)H and FAD. OMI is especially attractive for assessing tracheal health because OMI is label-free, and ciliary function is tightly linked to the levels of NAD(P)H and FAD. In this study, we apply widefield OMI to ex vivo mouse tracheae (n=6), and demonstrate that the optical redox ratio (fluorescence intensity of NAD(P)H divided by the intensity of FAD) is sensitive to changes in the cellular metabolism of the tracheal mucosa. We observed a 46% increase in the redox ratio 20 minutes after treatment with 10mM of sodium cyanide (p<0.001, 95% CI [40%, 52%]), an inhibitor of oxidative cellular respiration. In addition to being a proof-of-concept demonstration, Pseudomonas aeruginosa, an important cause of morbidity and mortality in CF patients and in the ICU, produces hydrogen cyanide. Our results support the development of minimally-invasive fiber-optic probes for in vivo monitoring of tracheal health.

Conference Presentation

Citation Download Citation

Daniel A. Gil, Joe T. Sharick, Ute A. Gamm, Michael A. Choma, and Melissa C. Skala "Functional optical imaging of tracheal health (Conference Presentation)", Proc. SPIE 10041, Optical Techniques in Pulmonary Medicine II, 100410O (19 April 2017); https://doi.org/10.1117/12.2252226

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Optical imaging

Medicine

Cyanide

Luminescence

Fiber optics

Hydrogen

In vivo imaging

Show All Keywords

Keywords/Phrases

Search In:

Publication Years