Optogenetic pacing in Drosophila models (Conference Presentation)

Penghe Wu; Airong Li; Jing Men; Rudolph E. Tans; Chao Zhou

doi:10.1117/12.2251653

19 April 2017 Optogenetic pacing in Drosophila models (Conference Presentation)

Penghe Wu, Airong Li, Jing Men, Rudolph E. Tans, Chao Zhou

Proceedings Volume 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System; 100430P (2017) https://doi.org/10.1117/12.2251653
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The Drosophila melanogaster shares many similarities with vertebrates in heart development. Comparison of heart structural and functional characteristic between male and female Drosophila melanogaster at different developmental stages is helpful to understand heart morphogenesis and function for different genders. And also, it opens up the possibility to uncover the role of sex-related genes in heart development. In this longitudinal study, we cultured and tracked dozens of individually labeled flies throughout their lifecycle. The heart characteristic was measured at different developmental stages during culturing. The gender of each individual fly was determined by adult stage so that the collected data of early stages could be classified to male or female group. We adapted a high-speed optical coherence microscopy (OCM) system with axial and transverse resolution of 2um and 4um, respectively, to perform non-invasive M-mode imaging at a frame rate of 132Hz in Drosophila heart at third instar larva, early pupa and adult stage. Based on those GPU processed M-mode OCM images, we segmented the fly heart region and then quantified the cardiac structural and functional parameters such as heart rate, heart chamber size and so on. Despite large variances of wild type Drosophila in terms of some cardiac characteristic, our results suggest that the heart rate is lower for male flies than for female flies, especially at third instar larva stage. The end diastolic area (EDA) and end systolic area (ESA) of the heart are both slightly larger in female flies than in male flies at larva and adult stage. In summary, we showed gender differences of wild type drosophila in heart functional and structural characteristic.

Conference Presentation

Citation Download Citation

Penghe Wu, Airong Li, Jing Men, Rudolph E. Tans, and Chao Zhou "Optogenetic pacing in Drosophila models (Conference Presentation)", Proc. SPIE 10043, Diagnosis and Treatment of Diseases in the Breast and Reproductive System, 100430P (19 April 2017); https://doi.org/10.1117/12.2251653

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KEYWORDS

Heart

Image segmentation

Imaging systems

Optogenetics

Electronic design automation

Image processing

Image resolution

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