Fluorescent tissue phantoms are useful constructs in tracking the daily performance of a fluorescence imaging system. However, fluorescence imaging systems vary according to intended use, such as with an endoscope, or a camera with wide field optics. They also vary in terms of spectral bandwidth, or the sensor. We present a method on how the fluorescence measurement results of a calibrated tissue phantom from two different fluorescence imaging systems can be compared. This demonstrates how tissue phantoms, when calibrated with units of optical radiance, can be used beyond a single optical system.
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