Automated imaging of cellular spheroids with selective plane illumination microscopy on a chip (Conference Presentation)

Petra Paiè; Andrea Bassi; Francesca Bragheri; Roberto Osellame

doi:10.1117/12.2253834

24 April 2017 Automated imaging of cellular spheroids with selective plane illumination microscopy on a chip (Conference Presentation)

Petra Paiè, Andrea Bassi, Francesca Bragheri, Roberto Osellame

Author Affiliations +

Proceedings Volume 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV; 100680I (2017) https://doi.org/10.1117/12.2253834
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Selective plane illumination microscopy (SPIM) is an optical sectioning technique that allows imaging of biological samples at high spatio-temporal resolution. Standard SPIM devices require dedicated set-ups, complex sample preparation and accurate system alignment, thus limiting the automation of the technique, its accessibility and throughput. We present a millimeter-scaled optofluidic device that incorporates selective plane illumination and fully automatic sample delivery and scanning. To this end an integrated cylindrical lens and a three-dimensional fluidic network were fabricated by femtosecond laser micromachining into a single glass chip. This device can upgrade any standard fluorescence microscope to a SPIM system. We used SPIM on a CHIP to automatically scan biological samples under a conventional microscope, without the need of any motorized stage: tissue spheroids expressing fluorescent proteins were flowed in the microchannel at constant speed and their sections were acquired while passing through the light sheet. We demonstrate high-throughput imaging of the entire sample volume (with a rate of 30 samples/min), segmentation and quantification in thick (100-300 μm diameter) cellular spheroids. This optofluidic device gives access to SPIM analyses to non-expert end-users, opening the way to automatic and fast screening of a high number of samples at subcellular resolution.

Conference Presentation

Citation Download Citation

Petra Paiè, Andrea Bassi, Francesca Bragheri, and Roberto Osellame "Automated imaging of cellular spheroids with selective plane illumination microscopy on a chip (Conference Presentation)", Proc. SPIE 10068, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, 100680I (24 April 2017); https://doi.org/10.1117/12.2253834

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KEYWORDS

Microscopy

Microscopes

Tissue optics

Biomedical optics

Femtosecond laser micromachining

Glasses

Image resolution

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