Label-free carbon particulates detection in bio (medical) settings (Conference Presentation)

Christian Steuwe; Hannelore Bové; Martin J. vandeVen; Marcel Ameloot; Maarten B. J. Roeffaers

doi:10.1117/12.2252656

24 April 2017 Label-free carbon particulates detection in bio (medical) settings (Conference Presentation)

Christian Steuwe, Hannelore Bové, Martin J. vandeVen, Marcel Ameloot, Maarten B. J. Roeffaers

Proceedings Volume 10069, Multiphoton Microscopy in the Biomedical Sciences XVII; 100691W (2017) https://doi.org/10.1117/12.2252656
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The adverse health effects of particulate matter exposure are a generally accepted concern. Dramatic statistical figures suggest that fine dust is a main environmental risk in Europe and can be held accountable for hundreds of thousands of deaths per year [1]. Locating and tracking these nanometer sized particles, however, is not straight forward: In epidemiological and toxicology research only measurements based on labels [2] such as radionuclide markers have been applied. In this paper we present a direct, label-free optical contrast mechanism to detect carbon nanoparticles immersed in aqueous environments [3]. The virtue of this technique is its ability to perform in body fluids such as urine but also in cells and tissues. The mechanism is based on white light (WL) generation upon illumination with femtosecond pulsed near-infrared and is therefore non-incandescence related. We demonstrate the technique in various biological settings with dry and suspended carbon black particles (CB), a widely used model compound for soot [4]. Our approach allows for the unequivocal localization of CB alongside of common fluorophores and markers and can be performed on multiphoton laser-scanning microscopy platforms, a system commonly available in research laboratories. [1] European Environment Agency (2015). Press release. [2] Kong et al. Int. J. Mol. Sci. 2013, 14, (11), 22529-22543 [3] Bové and Steuwe et al. Nano letters, 2016, (16) , pages 3173-3178 [4] Arnal et al. Combust. Sci. Technol. 2012, 184, (7-8), 1191-1206.

Conference Presentation

Citation Download Citation

Christian Steuwe, Hannelore Bové, Martin J. vandeVen, Marcel Ameloot, and Maarten B. J. Roeffaers "Label-free carbon particulates detection in bio (medical) settings (Conference Presentation)", Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, 100691W (24 April 2017); https://doi.org/10.1117/12.2252656

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Carbon

Particles

Multiphoton microscopy

Environmental sensing

Femtosecond phenomena

Nanoparticles

Radio optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years