Engineering of bacterial phytochromes for in vivo imaging (Conference Presentation)

Vladislav Verkhusha; Daria M. Shcherbakova; Andrii A. Kaberniuk; Mikhail Baloban

doi:10.1117/12.2254180

24 April 2017 Engineering of bacterial phytochromes for in vivo imaging (Conference Presentation)

Vladislav Verkhusha, Daria M. Shcherbakova, Andrii A. Kaberniuk, Mikhail Baloban

Proceedings Volume 10064, Photons Plus Ultrasound: Imaging and Sensing 2017; 1006404 (2017) https://doi.org/10.1117/12.2254180
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Genetically encoded probes with absorbance and fluorescence spectra within a near-infrared tissue transparency window are preferable for deep-tissue imaging. On the basis of bacterial phytochromes we engineered several types of near-infrared absorbing probes for photoacoustic tomography and fluorescent probes for purely optical imaging. They can be used as protein and cell labels and as building blocks for biosensors. The probes enabled imaging of tumors and metastases, protein-protein interactions, RNA visualization, detection of apoptosis, cellular metabolites, signaling pathways and cell proliferation. The developed probes allow non-invasive visualization of biological processes across scales, from super-resolution microscopy to tissue and whole-body animal imaging.

Conference Presentation

Citation Download Citation

Vladislav Verkhusha, Daria M. Shcherbakova, Andrii A. Kaberniuk, and Mikhail Baloban "Engineering of bacterial phytochromes for in vivo imaging (Conference Presentation)", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 1006404 (24 April 2017); https://doi.org/10.1117/12.2254180

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