Super-resolution atomic force photoactivated microscopy of biological samples (Conference Presentation)

Seunghyun Lee; Hyemin Kim; Seungjun Shin; Junsang Doh; Chulhong Kim

doi:10.1117/12.2251288

24 April 2017 Super-resolution atomic force photoactivated microscopy of biological samples (Conference Presentation)

Seunghyun Lee, Hyemin Kim, Seungjun Shin, Junsang Doh, Chulhong Kim

Proceedings Volume 10064, Photons Plus Ultrasound: Imaging and Sensing 2017; 1006425 (2017) https://doi.org/10.1117/12.2251288
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Optical microscopy (OM) and photoacoustic microscopy (PAM) have previously been used to image the optical absorption of intercellular features of biological cells. However, the optical diffraction limit (~200 nm) makes it difficult for these modalities to image nanoscale inner cell structures and the distribution of internal cell components. Although super-resolution fluorescence microscopy, such as stimulated emission depletion microscopy (STED) and stochastic optical reconstruction microscopy (STORM), has successfully performed nanoscale biological imaging, these modalities require the use of exogenous fluorescence agents, which are unfavorable for biological samples. Our newly developed atomic force photoactivated microscopy (AFPM) can provide optical absorption images with nanoscale lateral resolution without any exogenous contrast agents. AFPM combines conventional atomic force microscopy (AFM) and an optical excitation system, and simultaneously provides multiple contrasts, such as the topography and magnitude of optical absorption. AFPM can detect the intrinsic optical absorption of samples with ~8 nm lateral resolution, easily overcoming the diffraction limit. Using the label-free AFPM system, we have successfully imaged the optical absorption properties of a single melanoma cell (B16F10) and a rosette leaf epidermal cell of Arabidopsis (ecotype Columbia (Col-0)) with nanoscale lateral resolution. The remarkable images show the melanosome distribution of a melanoma cell and the biological structures of a plant cell. AFPM provides superior imaging of optical absorption with a nanoscale lateral resolution, and it promises to become widely used in biological and chemical research.

Conference Presentation

Citation Download Citation

Seunghyun Lee, Hyemin Kim, Seungjun Shin, Junsang Doh, and Chulhong Kim "Super-resolution atomic force photoactivated microscopy of biological samples (Conference Presentation)", Proc. SPIE 10064, Photons Plus Ultrasound: Imaging and Sensing 2017, 1006425 (24 April 2017); https://doi.org/10.1117/12.2251288

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KEYWORDS

Absorption

Microscopy

Atomic force microscopy

Tissue optics

Biomedical optics

Image resolution

Super resolution

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