Optofluidic time-stretch quantitative phase microscopy for high-throughput label-free single-cell analysis (Conference Presentation)

Baoshan Guo; Cheng Lei; Takuro Ito; Yiyue Jiang; Yasuyuki Ozeki; Keisuke Goda

doi:10.1117/12.2250824

24 April 2017 Optofluidic time-stretch quantitative phase microscopy for high-throughput label-free single-cell analysis (Conference Presentation)

Baoshan Guo, Cheng Lei, Takuro Ito, Yiyue Jiang, Yasuyuki Ozeki, Keisuke Goda

Author Affiliations +

Proceedings Volume 10074, Quantitative Phase Imaging III; 1007402 (2017) https://doi.org/10.1117/12.2250824
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

The ability to sift through a large heterogeneous population of cells is of paramount importance in a diverse range of biomedical and green applications. Furthermore, the capability of identifying various features of cells in a label-free manner is useful for high-throughput screening. Here we present optofluidic time-stretch quantitative phase microscopy for high-throughput label-free single-cell screening. This method is based on an integration of a hydrodynamic-focusing microfluidic chip, an optical time-stretch microscope for high-speed imaging with a spatial resolution of ~800 nm at a frame rate of ~10 million frames per second, and a digital image processor for image-based characterization, classification, and statistical analysis of biological cells such as blood cells and microalgae. It provides both the opacity (amplitude) and thickness (phase) content of every cell at a high throughput of ~10,000 cells per second. This method is expected to be effective for a diverse range of applications such as cancer detection and biofuel production.

Conference Presentation

Citation Download Citation

Baoshan Guo, Cheng Lei, Takuro Ito, Yiyue Jiang, Yasuyuki Ozeki, and Keisuke Goda "Optofluidic time-stretch quantitative phase microscopy for high-throughput label-free single-cell analysis (Conference Presentation)", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007402 (24 April 2017); https://doi.org/10.1117/12.2250824

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