CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume 10074 > Article
Presentation
24 April 2017 Three-dimensional refractive index and fluorescence tomography using structured illumination (Conference Presentation)
GwangSik Park, SeungWoo Shin, Kyoohyun Kim, YongKeun Park
Author Affiliations +
Proceedings Volume 10074, Quantitative Phase Imaging III; 1007415 (2017) https://doi.org/10.1117/12.2255702
Event: SPIE BiOS, 2017, San Francisco, California, United States
Abstract
Optical diffraction tomography (ODT) has been an emerging optical technique for label-free imaging of three-dimensional (3-D) refractive index (RI) distribution of biological samples. ODT employs interferometric microscopy for measuring multiple holograms of samples with various incident angles, from which the Fourier diffraction theorem reconstructs the 3-D RI distribution of samples from retrieved complex optical fields. Since the RI value is linearly proportional to the protein concentration of biological samples where the proportional coefficient is called as refractive index increment (RII), reconstructed 3-D RI tomograms provide precise structural and biochemical information of individual biological samples. Because most proteins have similar RII value, however, ODT has limited molecular specificity, especially for imaging eukaryotic cells having various types of proteins and subcellular organelles. Here, we present an ODT system combined with structured illumination microscopy which can measure the 3-D RI distribution of biological samples as well as 3-D super-resolution fluorescent images in the same optical setup. A digital micromirror device (DMD) controls the incident angle of the illumination beam for tomogram reconstruction, and the same DMD modulates the structured illumination pattern of the excitation beam for super-resolution fluorescent imaging. We first validate the proposed method for simultaneous optical diffraction tomographic imaging and super-resolution fluorescent imaging of fluorescent beads. The proposed method is also exploited for various biological samples.
Conference Presentation
© (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
GwangSik Park, SeungWoo Shin, Kyoohyun Kim, and YongKeun Park "Three-dimensional refractive index and fluorescence tomography using structured illumination (Conference Presentation)", Proc. SPIE 10074, Quantitative Phase Imaging III, 1007415 (24 April 2017); https://doi.org/10.1117/12.2255702
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
3D image processing
Refractive index
Biomedical optics
Diffraction
Proteins
Super resolution
Digital micromirror devices
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top