High-sensitivity chemical imaging for biomedicine by SRS microscopy (Conference Presentation)

Wei Min

doi:10.1117/12.2256239

24 April 2017 High-sensitivity chemical imaging for biomedicine by SRS microscopy (Conference Presentation)

Wei Min

Proceedings Volume 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II; 1007602 (2017) https://doi.org/10.1117/12.2256239
Event: SPIE BiOS, 2017, San Francisco, California, United States

Abstract

Innovations in spectroscopy principles and microscopy technology have significantly impacted modern biology and medicine. While most of the contemporary bio-imaging modalities harness electronic transition, nuclear spin or radioactivity, vibrational spectroscopy has not been widely used yet. Here we will discuss an emerging chemical imaging platform, stimulated Raman scattering (SRS) microscopy, which can enhance the otherwise feeble spontaneous Raman eight orders of magnitude by virtue of stimulated emission. When coupled with stable isotopes (e.g., deuterium and 13C) or bioorthogonal chemical moieties (e.g., alkynes), SRS microscopy is well suited for probing in vivo metabolic dynamics of small bio-molecules which cannot be labeled by bulky fluorophores. Physical principle of the underlying optical spectroscopy and exciting biomedical applications such as imaging lipid metabolism, protein synthesis, DNA replication, protein degradation, RNA synthesis, glucose uptake, drug trafficking and tumor metabolism will be presented.

Conference Presentation

Citation Download Citation

Wei Min "High-sensitivity chemical imaging for biomedicine by SRS microscopy (Conference Presentation)", Proc. SPIE 10076, High-Speed Biomedical Imaging and Spectroscopy: Toward Big Data Instrumentation and Management II, 1007602 (24 April 2017); https://doi.org/10.1117/12.2256239

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KEYWORDS

Microscopy

Imaging spectroscopy

Mode conditioning cables

Proteins

Biomedical optics

Spectroscopy

Biology

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