Characterization of E coli biofim formations on baby spinach leaf surfaces using hyperspectral fluorescence imaging

Hyunjeong Cho; Insuck Baek; Mirae Oh; Sungyoun Kim; Hoonsoo Lee; Moon S. Kim

doi:10.1117/12.2264826

4 May 2017 Characterization of E coli biofim formations on baby spinach leaf surfaces using hyperspectral fluorescence imaging

Hyunjeong Cho, Insuck Baek, Mirae Oh, Sungyoun Kim, Hoonsoo Lee, Moon S. Kim

Author Affiliations +

Proceedings Volume 10217, Sensing for Agriculture and Food Quality and Safety IX; 102170X (2017) https://doi.org/10.1117/12.2264826
Event: SPIE Commercial + Scientific Sensing and Imaging, 2017, Anaheim, CA, United States

Abstract

Bacterial biofilm formed by pathogens on fresh produce surfaces is a food safety concern because the complex extracellular matrix in the biofilm structure reduces the reduction and removal efficacies of washing and sanitizing processes such as chemical or irradiation treatments. Therefore, a rapid and nondestructive method to identify pathogenic biofilm on produce surfaces is needed to ensure safe consumption of fresh, raw produce. This research aimed to evaluate the feasibility of hyperspectral fluorescence imaging for detecting Escherichia.coli (ATCC 25922) biofilms on baby spinach leaf surfaces. Samples of baby spinach leaves were immersed and inoculated with five different levels (from 2.6x10⁴ to 2.6x10⁸ CFU/mL) of E.coli and stored at 4°C for 24 h and 48 h to induce biofilm formation. Following the two treatment days, individual leaves were gently washed to remove excess liquid inoculums from the leaf surfaces and imaged with a hyperspectral fluorescence imaging system equipped with UV-A (365 nm) and violet (405 nm) excitation sources to evaluate a spectral-image-based method for biofilm detection. The imaging results with the UV-A excitation showed that leaves even at early stages of biofilm formations could be differentiated from the control leaf surfaces. This preliminary investigation demonstrated the potential of fluorescence imaging techniques for detection of biofilms on leafy green surfaces.

Citation Download Citation

Hyunjeong Cho, Insuck Baek, Mirae Oh, Sungyoun Kim, Hoonsoo Lee, and Moon S. Kim "Characterization of E coli biofim formations on baby spinach leaf surfaces using hyperspectral fluorescence imaging", Proc. SPIE 10217, Sensing for Agriculture and Food Quality and Safety IX, 102170X (4 May 2017); https://doi.org/10.1117/12.2264826

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