Optimal design of dark field illumination optical system for the bacterial colony imaging and selection device

Jihong Zheng; Kangni Wang; Lu Liu; Xiaopeng Wei; Daoping Li; Rongfu Zhang; Songlin Zhuang

doi:10.1117/12.2267987

5 May 2017 Optimal design of dark field illumination optical system for the bacterial colony imaging and selection device

Jihong Zheng, Kangni Wang, Lu Liu, Xiaopeng Wei, Daoping Li, Rongfu Zhang, Songlin Zhuang

Author Affiliations +

Proceedings Volume 10209, Image Sensing Technologies: Materials, Devices, Systems, and Applications IV; 102090Z (2017) https://doi.org/10.1117/12.2267987
Event: SPIE Commercial + Scientific Sensing and Imaging, 2017, Anaheim, CA, United States

Abstract

Dark field illumination in high throughput colony selection device is reported in this paper. It improves the contrast of image in the premise of uniform illumination. Three important factors including angular distribution of light radiation, number of LED circle array and distance between the target and the LED circle source were analyzed, which affect illumination uniformity of dark field illumination. The simulation result shows that the illumination uniformity is 93.16% when the angular distribution of light radiation is 75°, the number of LED circle array is 3, and the distance between the target and the LED circle source is 61mm. In addition, Experimental system was set up in order to test designed dark field illumination, which matches with the project requirement and simulated results very well.

Citation Download Citation

Jihong Zheng, Kangni Wang, Lu Liu, Xiaopeng Wei, Daoping Li, Rongfu Zhang, and Songlin Zhuang "Optimal design of dark field illumination optical system for the bacterial colony imaging and selection device", Proc. SPIE 10209, Image Sensing Technologies: Materials, Devices, Systems, and Applications IV, 102090Z (5 May 2017); https://doi.org/10.1117/12.2267987

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