Analysis of off-axis incoherent digital holographic microscopy

Xiangyu Quan; Osamu Matoba; Yasuhiro Awatsuji

doi:10.1117/12.2264217

10 May 2017 Analysis of off-axis incoherent digital holographic microscopy

Xiangyu Quan, Osamu Matoba, Yasuhiro Awatsuji

Proceedings Volume 10219, Three-Dimensional Imaging, Visualization, and Display 2017; 102190U (2017) https://doi.org/10.1117/12.2264217
Event: SPIE Commercial + Scientific Sensing and Imaging, 2017, Anaheim, CA, United States

Abstract

Off-axis incoherent digital holography that enables single-shot three-dimensional (3D) distribution is introduced in the paper. Conventional fluorescence microscopy images 3D fields by sectioning, this prevents instant imaging of fast reactions of living cells. In order to realize digital holography from incoherent light, we adapted common path configuration to achieve the best temporal coherence. And by introducing gratings, we shifted the direction of each light to achieve off-axis interference. Simulations and preliminary experiments using LED light have confirmed the results. We expect to use this method to realize 3D phase imaging and fluorescent imaging at the same time from the same biological sample.

Conference Presentation

Citation Download Citation

Xiangyu Quan, Osamu Matoba, and Yasuhiro Awatsuji "Analysis of off-axis incoherent digital holographic microscopy", Proc. SPIE 10219, Three-Dimensional Imaging, Visualization, and Display 2017, 102190U (10 May 2017); https://doi.org/10.1117/12.2264217

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