Supercritical angle fluorescence as a tool to study the interaction between lipid bilayer and peptides

Valentin Dubois; Diana Serrano; Stefan Seeger

doi:10.1117/12.2267140

9 June 2017 Supercritical angle fluorescence as a tool to study the interaction between lipid bilayer and peptides

Valentin Dubois, Diana Serrano, Stefan Seeger

Proceedings Volume 10231, Optical Sensors 2017; 102312E (2017) https://doi.org/10.1117/12.2267140
Event: SPIE Optics + Optoelectronics, 2017, Prague, Czech Republic

Abstract

The understanding of processes occurring at the interface between two media are of prior importance in various fields of research, from material sciences to biology. A custom-made microscope objective based on the supercritical angle technique was developed in our group, allowing to probe these interfacial events by carrying out surface-sensitive and low invasive spectroscopy of aqueous samples. A biological example of particular interest is the comprehension of neurodegenerative diseases which seem caused by the interaction of specific peptides with the membrane of the neurons. Taking advantage of our optical setup, we used supercritical angle fluorescence spectroscopy to specifically monitor the interaction between a supported lipid bilayer (SLB) and the Amyloid β peptide, notably responsible of the Alzheimer disease. Different forms of the peptide (40 and 42 amino acids composition) were tested and the interfacial fluorescence measured to get information about the lipid integrity and mobility. The adsorption of the peptide was also characterized in terms of kinetic and affinity.

Citation Download Citation

Valentin Dubois, Diana Serrano, and Stefan Seeger "Supercritical angle fluorescence as a tool to study the interaction between lipid bilayer and peptides", Proc. SPIE 10231, Optical Sensors 2017, 102312E (9 June 2017); https://doi.org/10.1117/12.2267140

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