DMD based digital speckle illumination for high resolution imaging

Anant Shinde; Ayush Mishra; Sandeep M. Perinchery; V. Matham Murukeshan

doi:10.1117/12.2269570

13 June 2017 DMD based digital speckle illumination for high resolution imaging

Anant Shinde, Ayush Mishra, Sandeep M. Perinchery, V. Matham Murukeshan

Proceedings Volume 10449, Fifth International Conference on Optical and Photonics Engineering; 104490J (2017) https://doi.org/10.1117/12.2269570
Event: Fifth International Conference on Optical and Photonics Engineering, 2017, Singapore, Singapore

Abstract

Spatially non-uniform illumination patterns have shown significant potential to improve the imaging. Recent developments in the patterned illumination microscopy have demonstrated that the use of an optical speckle as an illumination pattern significantly improves the imaging resolution at the same time reducing the computational overheads. We present a DMD based method for generation of digital speckle pattern. The generated digital speckle and uniform white light illumination are used as two illuminations to acquire images. The image reconstruction algorithm for blind structured illumination microscopy is used to get the high resolution image. Our approach does not require any calibration step or stringent control of the illumination, and dramatically simplifies the experimental set-up.

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Anant Shinde, Ayush Mishra, Sandeep M. Perinchery, and V. Matham Murukeshan "DMD based digital speckle illumination for high resolution imaging", Proc. SPIE 10449, Fifth International Conference on Optical and Photonics Engineering, 104490J (13 June 2017); https://doi.org/10.1117/12.2269570

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KEYWORDS

Image resolution

Biomedical optics

Microscopy

Speckle pattern

Calibration

Digital micromirror devices

Image processing

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