Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

RBCs as microlenses: wavefront analysis and applications

[+] Author Affiliations
Francesco Merola, Lisa Miccio, Pasquale Memmolo, Martina Mugnano, Pietro Ferraro

Istituto di Scienze Applicata e Sistemi Intelligenti-CNR (Italy)

Álvaro Barroso, Cornelia Denz

Westfälische Wilhelms-Univ. Münster (Germany)

Proc. SPIE 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III, 1033311 (June 26, 2017); doi:10.1117/12.2272042
Text Size: A A A
From Conference Volume 10333

  • Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III
  • Pietro Ferraro; Simonetta Grilli; Monika Ritsch-Marte; Christoph K. Hitzenberger
  • Munich, Germany | June 25, 2017

abstract

Developing the recently discovered concept of RBCs as microlenses, we demonstrate further applications in wavefront analysis and diagnostics. Correlation between RBC’s morphology and its behavior as a refractive optical element has been established. In fact, any deviation from the healthy RBC morphology can be seen as additional aberration in the optical wavefront passing through the cell. By this concept, accurate localization of focal spots of RBCs can become very useful in blood disorders identification. Moreover, By modelling RBC as bio-lenses through Zernike polynomials it is possible to identify a series of orthogonal parameters able to recognise RBC shapes. The main improvement concerns the possibility to combine such parameters because of their independence conversely to standard image-based analysis where morphological factors are dependent each-others. We investigate the three-dimensional positioning of such focal spots over time for samples with two different osmolarity conditions, i.e. discocytes and spherocytes. Finally, Zernike polynomials wavefront analysis allows us to study the optical behavior of RBCs under an optically-induced mechanical stress. Detailed wavefront analysis provides comprehensive information about the aberrations induced by the deformation obtained using optical tweezers. This could open new routes for analyzing cell elasticity by examining optical parameters instead of direct but with low resolution strain analysis, thanks to the high sensitivity of the interferometric tool. © (2017) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Francesco Merola ; Álvaro Barroso ; Lisa Miccio ; Pasquale Memmolo ; Martina Mugnano, et al.
" RBCs as microlenses: wavefront analysis and applications ", Proc. SPIE 10333, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials III, 1033311 (June 26, 2017); doi:10.1117/12.2272042; http://dx.doi.org/10.1117/12.2272042


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.