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Proceedings Article

Roles of dynamin-related protein 1 in the regulation of mitochondrial fission and apoptosis in response to UV stimuli

[+] Author Affiliations
Zhenzhen Zhang, Jie Feng

MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ. (China)

Shengnan Wu

MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ.. (China)

Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000G (February 10, 2011); doi:10.1117/12.874292
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From Conference Volume 7900

  • Biophotonics and Immune Responses VI
  • Wei R. Chen
  • San Francisco, California, USA | January 22, 2011

abstract

Mitochondria are dynamic structures that frequently divide and fuse with one another to form interconnecting network. This network disintegrates into punctiform organelles during apoptosis. However, it remains unclear whether this event has a significant impact on the rate of cell death or only accompanies apoptosis as an epiphenomenon. In this study, we investigate the role of dynamin-related protein 1 (Drp1), a large GTPase that mediates outer mitochondrial membrane fission, in mitochondrial morphology and apoptosis in response to UV irradiation in human lung adenocarcinoma cells (ASTC-a-1) and HeLa cells. Using time-lapse fluorescent imaging, we find that Drp1 primarily distributes in cytosol under physiological conditions. After UV treatment, Drp1 translocates from cytosol to mitochondria, indicating the enhancement of Drp1 mitochondrial accumulation. Down-regulation of Drp1 by shRNA inhibits UV-induced apoptosis. Our results suggest that Drp1 is involved in the regulation of transition from a reticulo-tubular to a punctiform mitochondrial phenotype and mitochondrial fission plays an important role in UV-induced apoptosis.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Zhenzhen Zhang ; Jie Feng and Shengnan Wu
"Roles of dynamin-related protein 1 in the regulation of mitochondrial fission and apoptosis in response to UV stimuli", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000G (February 10, 2011); doi:10.1117/12.874292; http://dx.doi.org/10.1117/12.874292


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