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Proceedings Article

Effect of low power laser irradiation on macrophage phagocytic capacity

[+] Author Affiliations
Cuixia Lu, Sheng Song, Yu Tang, Feifan Zhou

MOE Key Lab. of Laser Life Science & Institute of Laser Life Science, South China Normal Univ. (China)

Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000H (February 10, 2011); doi:10.1117/12.874325
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From Conference Volume 7900

  • Biophotonics and Immune Responses VI
  • Wei R. Chen
  • San Francisco, California, USA | January 22, 2011

abstract

Phagocytosis and subsequent degradation of pathogens by macrophages play a pivotal role in host innate immunity in mammals. Laser irradiation has been found to produce photobiological effects with evidence of interference with immunological functions. However, the effects of laser on the immune response have not been extensively characterized. In this study, we focused our attention on the effects of He-Ne laser on the phagocytic activity of macrophages by using flow cytometry (FCM). After irradiating at fluence of 0, 1, 2 J/cm2 with He-Ne laser (632.8 nm, 3mw), the cells were incubated with microsphere and then subjected to FACS analysis. The results showed that Low-power laser irradiation (LPLI) leads to an increase in phagocytosis on both mouse peritoneal macrophages and the murine macrophage-like cell line RAW264.7. In addition, we demonstrated that LPLI increased phagocytosis of microsphere in a dose-dependent manner, reaching a maximum at fluence of 2 J/cm2. Taken together, our results indicated that Low-power laser irradiation with appropriate dosage can enhance the phagocytosis of macrophage, and provided a theoretical base for the clinical use of the He-Ne laser.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Cuixia Lu ; Sheng Song ; Yu Tang and Feifan Zhou
"Effect of low power laser irradiation on macrophage phagocytic capacity", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000H (February 10, 2011); doi:10.1117/12.874325; http://dx.doi.org/10.1117/12.874325


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