Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells

Huai-na Yu; Ying-ying Lu; Tong-sheng Chen

doi:10.1117/12.876977

10 February 2011 Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells

Huai-na Yu, Ying-ying Lu, Tong-sheng Chen

Proceedings Volume 7900, Biophotonics and Immune Responses VI; 79000J (2011) https://doi.org/10.1117/12.876977
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Dihydroartemisinin (DHA), a first-line anti-malarial drug with low toxicity, has been shown to possess promising anticancer activities and induce cancer cell death through apoptotic pathway, but the molecular mechanisms are not well understood. In this paper, we focus on whether Bad, a BH3-only pro-apoptotic protein, is involved in apoptotic cell death in DHA-treated human lung adenocarcinoma (ASTC-a-1) cells. Confocal fluorescence microscope imaging was used to monitor the temporal and spatial distribution of Bad in single living cells. Our results indicate that Bad is still located in cytoplasm and does not translocate to mitochondria after treatment with DHA for 24 h, while only a small proportion of Bad located in cytoplasm in the STS-treated cells for 6 h. These results show for the first time that Bad is not involved in DHA-induced apoptosis in ASTC-a-1 cells, which could give more evidence for the molecular mechanisms of apoptosis induced by DHA.

Citation Download Citation

Huai-na Yu, Ying-ying Lu, and Tong-sheng Chen "Bad is not involved in DHA-induced apoptosis in human lung adenocarcinoma ASTC-a-1 cells", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 79000J (10 February 2011); https://doi.org/10.1117/12.876977

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