Immunohistochemical analysis of immune response in breast cancer and melanoma patients after laser immunotherapy

Robert E. Nordquist; Shelly L. Bishop; Halie Ferguson; Melville B. Vaughan; Jessnie Jose; Katherine Kastl; Long Nguyen; Xiaosong Li; Hong Liu; Wei R. Chen

doi:10.1117/12.874875

23 February 2011 Immunohistochemical analysis of immune response in breast cancer and melanoma patients after laser immunotherapy

Robert E. Nordquist, Shelly L. Bishop, Halie Ferguson, Melville B. Vaughan, Jessnie Jose, Katherine Kastl, Long Nguyen, Xiaosong Li, Hong Liu, Wei R. Chen

Author Affiliations +

Proceedings Volume 7900, Biophotonics and Immune Responses VI; 790007 (2011) https://doi.org/10.1117/12.874875
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Laser immunotherapy (LIT) has shown great promise in pre-clinical studies and preliminary clinical trials. It could not only eradicate treated local tumors but also cause regression and elimination of untreated metastases at distant sites. Combining a selective photothermal therapy with an active immunological stimulation, LIT can induce systemic anti-tumor immune responses. Imiquimod (IMQ), a toll-like receptor agonist, was used for the treatment of late-stage melanoma patients and glycated chitosan (GC), a biological immunological modulator, was used for the treatment of late-stage breast cancer patients, in combination of irradiation of a near-infrared laser light. To observe the immunological changes before and after LIT treatment, the pathological tissues of melanoma and breast cancer patients were processed for immunohistochemical analysis. Our results show that LIT changed the expressions of several crucial T cell types. Specifically, we observed significant decreases of CD3+ T-cells and a significant increase of CD4+,CD8+, and CD68+ T-cells in the tumor samples after LIT treatment. While not conclusive, our study could shed light on one the possible mechanisms of anti-tumor immune responses induced by LIT. Further studies will be conducted to identify immunological biomarkers associated with LIT-induced clinical response.

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Robert E. Nordquist, Shelly L. Bishop, Halie Ferguson, Melville B. Vaughan, Jessnie Jose, Katherine Kastl, Long Nguyen, Xiaosong Li, Hong Liu, and Wei R. Chen "Immunohistochemical analysis of immune response in breast cancer and melanoma patients after laser immunotherapy", Proc. SPIE 7900, Biophotonics and Immune Responses VI, 790007 (23 February 2011); https://doi.org/10.1117/12.874875

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