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Proceedings Article

Autofluorescence ratio imaging of human colonic adenomas

[+] Author Affiliations
Katsuichi Imaizumi

Kyoto Prefectural Univ. of Medicine (Japan) and Olympus Medical Systems Corp. (Japan)

Yoshinori Harada, Naoki Wakabayashi, Yoshihisa Yamaoka, Ping Dai, Hideo Tanaka, Tetsuro Takamatsu

Kyoto Prefectural Univ. of Medicine (Japan)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020E (February 10, 2011); doi:10.1117/12.874869
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From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

Recently autofluorescence imaging (AFI) endoscopy, visualizing tissue fluorescence in combination with reflected light, has been adopted as a technique for detecting neoplasms in the colon and other organs. However, autofluorescence colonoscopy is not infallible, and improvement of the detection method can be expected to enhance the performance. Colonic mucosa contains metabolism-related fluorophores, such as reduced nicotinamide adenine dinucleotide, which may be useful for visualizing neoplasia in autofluorescence endoscopy. We examined sliced cross-sections of endoscopically resected tubular adenomas under a microscope. Fluorescence images acquired at 365-nm excitation (F365ex) and 405-nm excitation (F405ex), and reflectance images acquired at 550 nm (R550) were obtained. Fluorescence ratio (F365ex/F405ex) images and reflectance/fluorescence ratio (R550/F405ex) images were calculated from the acquired images. The fluorescence ratio images could distinguish adenomatous mucosa from normal mucosa more clearly than the reflectance/fluorescence ratio images. The results showed that the autofluorescence ratio imaging is a potential technique for increasing the diagnostic power of autofluorescence endoscopy.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Katsuichi Imaizumi ; Yoshinori Harada ; Naoki Wakabayashi ; Yoshihisa Yamaoka ; Ping Dai, et al.
"Autofluorescence ratio imaging of human colonic adenomas", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020E (February 10, 2011); doi:10.1117/12.874869; http://dx.doi.org/10.1117/12.874869


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