Autofluorescence ratio imaging of human colonic adenomas

Katsuichi Imaizumi; Yoshinori Harada; Naoki Wakabayashi; Yoshihisa Yamaoka; Ping Dai; Hideo Tanaka; Tetsuro Takamatsu

doi:10.1117/12.874869

10 February 2011 Autofluorescence ratio imaging of human colonic adenomas

Katsuichi Imaizumi, Yoshinori Harada, Naoki Wakabayashi, Yoshihisa Yamaoka, Ping Dai, Hideo Tanaka, Tetsuro Takamatsu

Proceedings Volume 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX; 79020E (2011) https://doi.org/10.1117/12.874869
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Recently autofluorescence imaging (AFI) endoscopy, visualizing tissue fluorescence in combination with reflected light, has been adopted as a technique for detecting neoplasms in the colon and other organs. However, autofluorescence colonoscopy is not infallible, and improvement of the detection method can be expected to enhance the performance. Colonic mucosa contains metabolism-related fluorophores, such as reduced nicotinamide adenine dinucleotide, which may be useful for visualizing neoplasia in autofluorescence endoscopy. We examined sliced cross-sections of endoscopically resected tubular adenomas under a microscope. Fluorescence images acquired at 365-nm excitation (F_365ex) and 405-nm excitation (F_405ex), and reflectance images acquired at 550 nm (R₅₅₀) were obtained. Fluorescence ratio (F_365ex/F_405ex) images and reflectance/fluorescence ratio (R₅₅₀/F_405ex) images were calculated from the acquired images. The fluorescence ratio images could distinguish adenomatous mucosa from normal mucosa more clearly than the reflectance/fluorescence ratio images. The results showed that the autofluorescence ratio imaging is a potential technique for increasing the diagnostic power of autofluorescence endoscopy.

Citation Download Citation

Katsuichi Imaizumi, Yoshinori Harada, Naoki Wakabayashi, Yoshihisa Yamaoka, Ping Dai, Hideo Tanaka, and Tetsuro Takamatsu "Autofluorescence ratio imaging of human colonic adenomas", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020E (10 February 2011); https://doi.org/10.1117/12.874869

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KEYWORDS

Luminescence

Tumors

Endoscopy

Bandpass filters

Visualization

Reflectivity

CCD cameras

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