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Proceedings Article

Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles

[+] Author Affiliations
Jae Youn Hwang

Cedars-Sinai Medical Ctr. (USA) and Univ. of Southern California, Los Angeles (USA)

Zeev Gross

Beckman Research Institute (USA) and Technion-Israel Institute of Technology (Israel)

Harry B. Gray

Beckman Research Institute (USA)

Lali K. Medina-Kauwe

Cedars-Sinai Medical Ctr. (USA) and Univ. of California, Los Angeles (USA)

Daniel L. Farkas

Univ. of Southern California (USA) and Spectral Molecular Imaging, Inc. (USA)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020F (February 28, 2011); doi:10.1117/12.877780
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From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

We report the feasibility of tumor detection and delineation in vivo using multimode optical imaging of targeted gallium corrole (HerGa). HerGa is highly effective for targeted HER2+ tumor elimination in vivo, and it emits intense fluorescence. These unique characteristics of HerGa prompted us to investigate the potential of HerGa for tumor detection and delineation, by performing multimode optical imaging ex vivo and in vivo; the imaging modes included fluorescence intensity, spectral (including ratiometric), lifetime, and two-photon excited fluorescence, using our custombuilt imaging system. While fluorescence intensity imaging provided information about tumor targeting capacity and tumor retention of HerGa, ratiometric spectral imaging offered more quantitative and specific information about HerGa location and accumulation. Most importantly, the fluorescence lifetime imaging of HerGa allowed us to discriminate between tumor and non-tumor regions by fluorescence lifetime differences. Finally, two-photon excited fluorescence images provided highly resolved and thus topologically detailed information around the tumor regions where HerGa accumulates. Taken together, the results shown in this report suggest the feasibility of tumor detection and delineation by multimode optical imaging of HerGa, and fluorescent chemotherapy agents in general. Specifically, the multimode optical imaging can offer complementary and even synergetic information simultaneously in the tumor detection and delineation by HerGa, thus enhancing contrast.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Jae Youn Hwang ; Zeev Gross ; Harry B. Gray ; Lali K. Medina-Kauwe and Daniel L. Farkas
"Multimode optical imaging for translational chemotherapy: in vivo tumor detection and delineation by targeted gallium corroles", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79020F (February 28, 2011); doi:10.1117/12.877780; http://dx.doi.org/10.1117/12.877780


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