Full Content is available to subscribers

Subscribe/Learn More  >
Proceedings Article

Quantum cascade laser-based replacement for FTIR microscopy

[+] Author Affiliations
Miles J. Weida, Brandon Yee

Daylight Solutions, Inc. (USA)

Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021C (February 10, 2011); doi:10.1117/12.873954
Text Size: A A A
From Conference Volume 7902

  • Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX
  • Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif
  • San Francisco, California, USA | January 22, 2011

abstract

Infrared (IR) microscopy has shown itself to be an important diagnostic tool for tissue analysis. To date, the main tool for performing IR microscopy has been the Fourier transform infrared (FTIR) microscope. FTIR microscopes utilize incandescent bulbs for light sources, and require cryogenically cooled detectors for the weak, optically poor probe signals. Image acquisition times can be tens of minutes even for sophisticated instruments, and the size and cost of FTIR microscopes precludes their broader clinical use. The development of broadly tunable, external cavity quantum cascade lasers (ECqcLTM) has created an ideal light source for IR microscopy. Spectrally brilliant probe beams that are diffraction limited, with intensities many orders of magnitude higher than incandescent sources, can be generated from compact, room temperature ECqcLTM devices. Moreover, the increase in intensity allows the use of room temperature microbolometer focal plane arrays (FPAs) for detection. The combination of ECqcLsTM and microbolometer FPAs opens the possibility of producing low cost, compact, room temperature IR microscopes with acquisition speeds thirty times that of state-of-the-art FTIR microscopes. The present study explores the challenges of creating this new generation of IR microscopes, and demonstrates the capabilities of the technology.

© (2011) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation

Miles J. Weida and Brandon Yee
"Quantum cascade laser-based replacement for FTIR microscopy", Proc. SPIE 7902, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IX, 79021C (February 10, 2011); doi:10.1117/12.873954; http://dx.doi.org/10.1117/12.873954


Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).

Figures

Tables

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Proceeding
Sign in or Create a personal account to Buy this proceeding ($15 for members, $18 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.